Whole Plasmid Sequencing
Complete and accurate whole plasmid sequencing from 2.5 kb to 30 kb in length. Deliverables include sequence in TXT and FASTA file format, plus a fully annotated plasmid map.
Nanopore sequencing is a unique, scalable technology that enables direct, real-time analysis of long DNA or RNA fragments. It works by monitoring changes to an electrical current as nucleic acids are passed through a protein nanopore. The resulting signal is decoded to provide the specific DNA or RNA sequence.
In April of 2023, Poochon Scientific and Quintarabio joined forces to offer the area’s first 24-hour Nanopore Whole plasmid sequencing services. Through preferred pick-up and various drop box locations, Poochon offers convenient sample submission options for local clients. Quintarabio’s easy ordering platform allows for simple order processing and results.
To register an account and place an order, you will be redirected to Quintarabio’s website.
Complete and accurate whole plasmid sequencing from 2.5 kb to 30 kb in length. Deliverables include sequence in TXT and FASTA file format, plus a fully annotated plasmid map.
Addition to the standard whole plasmid sequence result, the predicted sequence provided will be automatically assembled with the whole plasmid sequence result sequence to save your time and budget.
Sequencing of clonal linear DNA prepared by PCR or LCR from 2 kb to 30 kb in length. Re-assemble the raw reads and align them against each other to generate a high-accuracy linear consensus sequence.
Full-length sequencing of double-stranded linear or circular DNA, between 500 bp and 300 kb in length. Amplification-free long-read sequencing library is constructed and sequenced using a dedicated R10.4.1 flow cell with > 99% accuracy.
Plasmid DNA or ≥ 2kb PCR
Up to 30kb Plasmid
1.8 ≤OD 260/280 ≤ 1.95
5μl, 10μl preferred
100-200 ng/μl